Biology Topic 1: Cell Biology
71 flashcards · Biology · by Marli Williams
Eukaryotic Cells
Has genetic material in a nucleus
Examples of Eukaryotic Cells
Animal Cell Plant Cell
Size of Eukaryotic Cells
10 to 100 μm (micrometers)
Prokaryotic Cells
Has genetic material in cytoplasm - a single loop of DNA, possibly some additional plasmids
Examples of Prokaryotic Cells
Bacteria
Size of Prokaryotic Cells
1 μm
Order of Magnitude
Order of getting x10 bigger 1 Tm - 1 x 1012 m [Tera] 1 Gm - 1 x 109 m [Giga] 1 Mm - 1 x 106 m [Mega] 1 km - 1 x 103 m [Kilo] 1 dm - 1 x 10-1 m [deci] 1 cm - 1 x 10-2 m [centi] 1 mm - 1 x 10-3 m [milli] 1 μm - 1 x 10-6 m [micro] 1 nm - 1 x 10-9 m [nano] 1 pm - 1 x 10-12 m [pico]
Cell Membrane
Controls what goes in and out of the cell
Cytoplasm
Jelly-like substance where chemical reactions occur in the cell
Nucleus
Contains DNA - which controls activity of the cell
Mitochondria
Where respiration takes place in the cell
Ribosomes
Site of protein synthesis
Cell wall
Supports cell, defines its shape - made of cellulose
Chloroplasts
Contains chlorophyll which absorbs light energy for photosynthesis
Permanent Vacuole
Used for storage and supports shape of the cell Made of cell sap - a solution of soluble sugars and salts
Explain how to prepare microscopy slide.
Place Specimen on the slide Add a stain like iodine Place a cover slip on top - careful not to create air bubbles
Required practical: Observing specimen using a light microscope
Prepare the slide adding a stain and being careful not to create air bubbles Put slide on the stage and start with the lowest magification Adjust the coarse lens to see the specimen more clearly Then adjust the fine lens to get a clear image Increase the magnification by using a different objective lens
Specialised Cells
Cell that are adapted to carry out a particular function Cell specialise by undergoing Differentiation
Nerve Cell Functions and Adaptations
Specialised to transmit electrical signals quickly within the body. Has a cell body where most sub-cellular structures are located Has dendrites to receive signals from other nerve cells, muscles and glands Has a long axon to send signals over a long distance Has Myelin sheath which insulates the electrical signals that travel
Muscle Cell Functions and Adaptations
Specialised to contract/squeeze to cause movement Has layers of protein filaments which slide over each other causing a muscle contraction Has lots of mitochondria Has stores of glycogen (stores of glucose for respiration)
Sperm Cell Functions and Adaptations
Specialised to carry the male's DNA to the egg cell for sexual reproduction Has a streamlined head and long tail to aid in swimming Has half the normal number of chromosomes (23) Has digestive enzymes in the acrosome within the head to break down the outer layer of egg cell Has lots of Mitochondria
Root Hair Cell Functions and Adaptations
Function: absorption of water and mineral ions from soil Has root hairs which increase the surface area - increasing water/mineral intake Has a large and concentrated permanent vacuole which increase water though a high concentration gradient Has thin walls for shorter diffusion distance Has no chloroplasts Has lots of Mitochondria for active transport
Xylem Functions and Adaptations
Specialised to transport water and mineral ions Dead, empty cells to allow free passage of water Strengthened by Ligin to withstand water pressure
Phloem Functions and Adaptations
Specialised to transport sugars and amino acids Living cells are joined end to end with sieve plates so nutrients can flow in both directions Has few sub-cellular structures to aid the flow of materials
Importance of Cell differentiation
For cells to become functional to therefore develop into a functional organism
When do Animal and Plant cells differentiate?
Animal Cells: At an early stage Mature Animal Cells only differentiate for repair and replacement Plant Cells: At any point through the plant's life
Microscopy Development
Light microscope was first developed in 17th century Over time the magnification and resolution has increased Electron microscopes were developed in the early 1900s Electron microscopes have higher magnification and resolution that light ones This means that it can be used to study cells in much finer detail. This has enabled biologists to see and understand many more sub-cellular structures.
Define Resolution
The shortest distance between two points on a specimen that can still be distinguished by the observer or camera system as separate entities.
Magnification equation
Magnification = Size of image / Actual size of specimen
Required Practical: Culturing Microorganisms
Independent Variable: Type of Antiseptic used Dependent Variable: Clear zone of Bacteria Control Variables: Sterile water
Why is Sterile water used as an control for Required practical 2?
To ensure that any differences in bacterial growth observed can be attributed to the presence of the antiseptic or antibiotic used and not some other factor (such as the paper discs for example)
Binary Fission
Simple Cell division which bacteria use
How fast do Bacteria reproduce?
Once every 20 minutes if they have enough nutrients and a suitable temperature.
Where can bacteria be cultured?
Bacteria can be grown in a nutrient broth solution or as colonies on an agar gel plate.
What are uncontaminated cultures used for
Investigating the action of disinfectants and antibiotics.
Why petri dishes and culture media must be sterilised before use?
The culture could be contaminated and have to compete with other organisms for space and food
Why must inoculating loops be sterilised by passing them through a flame?
To make sure any organism on them are killed to prevent contaminating the culture
Why must the lid of the Petri dish should be secured with adhesive tape and stored upside down?
To stop airborne microorganisms from contaminating the culture To prevent condensation from lid to land on the agar surface
Why must (in school laboratories) cultures should generally be incubated at 25°C.
Optimum temperature is 37℃ (body temperature) This makes the bacteria more dangerous as it could infect students.
Chromosomes
Contained within the nucleus and contains DNA 46 Chromosomes (23 pairs) in a human body cell
Mitosis
One set of chromosomes is pulled to each end of the cell and the nucleus divides.
The Cell Cycle
Cell increases in size and number of subcellular structures (like ribosomes and mitochondria) DNA replicates to form two copies of each chromosome Mitosis (One set of chromosomes is pulled to each end of the cell and the nucleus divides) The cytoplasm and cell membrane divide to form two identical daughter cells
Why is cell division by Mitosis important?
For the growth and development of multicellular organisms.
Stem Cell
A stem cell is an undifferentiated cell of an organism which is capable of giving rise to many more cells of the same type, and from which certain other cells can arise from differentiation.
Embryonic Stem Cells
Stem cells found in the early stages of organism development - are not found in mature organisms Function: To form all different cells to create a functional organism. (healthy baby)
Adult bone marrow
Unspecialised cells which can differentiate into certain cells throughout the life of the organism like blood cells Function: To replace cells lost via damage For growth
Plant meristem tissue
Unspecialised cells which can differentiate throughout the life of the plant Function: For growth and repair
What can Embryonic Stem Cells be used for?
They can be cloned and made to differentiate into most different types of human cells.
Treatment with stem cells could help with what diseases?
Diabetes - insulin resistance / little insulin production Paralysis - damaged nerve cells
Therapeutic Cloning
An embryo is produced with the same genes as the patient. Stem cells from the embryo are not rejected by the patient’s body so they may be used for medical treatment.
Cons of Stem Cells
Risk of transfer of viral infections Ethical objections
Stem cells from meristems in plants can be used to produce clones of plants quickly and economically. This means: Rare species can be cloned to protect from extinction. Crop plants with special features such as disease resistance can be cloned to produce large numbers of identical plants for farmers.
Why is the fact plants can be cloned useful?
Diffusion
The spreading out of the particles of any substance in solution, or particles of a gas, resulting in a net movement from an area of higher concentration to an area of lower concentration.
Factors of Diffusion Rate
The difference in concentrations (concentration gradient) The temperature The surface area of the membrane.
How does the difference in concentrations (concentration gradient) effect the rate of diffusion?
The greater the difference on concentration between two regions - faster the overall rate of diffusion
How does the temperature effect the rate of diffusion?
Higher temperature equals higher net movement of particles due to move energy
How does surface area of the membrane effect the rate of diffusion?
More space for the substances to diffuse through
Why do single-celled organisms have a relatively high surface area to volume ratio?
To allows sufficient transport of molecules into and out of the cell to meet the needs of the organism.
Why do multicellular organisms need exchange surfaces and transport systems?
Multicellular organisms need exchange surfaces and a transport system to gain sufficient diffusion / active transport / osmosis rates due to their relatively small SA to volume ratio. This is to allow for the sufficient transport of molecules into and out of the cells to meet the needs of the multicellular organism.
In multicellular organisms, surfaces and organ systems are specialised for exchanging materials. This is to allow sufficient molecules to be transported into and out of cells for the organism’s needs. The effectiveness of an exchange surface is increased by: having a large surface area a membrane that is thin, to provide a short diffusion path (in animals) having an efficient blood supply (in animals, for gaseous exchange) being ventilated.
Why do multicellular organisms need exchange surfaces and transport systems?
Small Intestine
Function: To absorb soluble food molecules into the blood Adaptations: Has a large SA via Villi Each villius has a good blood supply - maintaining a stable concentration gradient One cell thick - decreases diffusion distance
Lungs
Function: To exchange oxygen and carbon dioxide Adaptations: Millions of Alveoli - large SA Good ventilation Alveoli are one cell thick Alveoli have a good blood supply
Gills
Function: To exchange oxygen and carbon dioxide Adaptations: Each gill is made of filaments with projections called lamellae increasing SA Good blood supply that flows in the opposite direction of water - maintains a concentration gradient
Roots
Function: Absorption of Water and mineral ions Adaptations: Root hair cells with root hair projections increasing SA Lots of Mitochondria for active transport Concentrated via cell sap - maintaining a water concentration gradient
Leaves
Function: Gas Exchange Adaptations: Stomata allows airs to circulate in the leaf - Good ventilation Air gaps within the leaf - decreasing diffusion distance
Osmosis
The diffusion of water from a dilute solution to a concentrated solution through a partially permeable membrane.
Required Practical: Effects of Solutions on Plant Tissue
Independent Variable: Concentration of salt or sucrose within the solution Dependent Variable: Percentage Change in Mass Control Variables: Type and volume of solute in solution, temperature, and time
Why is percentage change in mass used as a dependent variable?
To make fair comparisons of the change of mass.
The change in mass when the tissue is more concentrated in water is decrease - water is lost. The change in mass when the tissue is less concentrated in water is increase - water is taken in. The change in mass when the tissue has the same concentration of water is no change.
Active Transport
Movement of substances from a more dilute solution to a more concentrated solution (against a concentration gradient). This requires energy from respiration.
Examples of Active Transport
Root hair cells - mineral ions from soil Gut - sugar molecules into the blood